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KMID : 0380620170490020215
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 2 p.215 ~ p.221
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Biological activity of water-soluble polysaccharides from Cedrela sinensis according to extraction methods
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Oh Min-Hui
Hwang Yeo-Jin
Yoon Kyung-Young
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Abstract
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The biological activity of water-soluble polysaccharide (WSP) fractions extracted from Cedrela sinensis was examined in this study. Cedrela sinensis was extracted using hot water, ultrasonication, and enzymes (Viscozyme, Shearzyme) and precipitated using ethanol to produce crude polysaccharides. The yield (3.51%) and total polysaccharide content (28.03 g/100 g) of WSP extracted using Shearzyme (WSPs) were highest compared to other extracts. The antioxidant activity of WSP extracted using hot water was highest and had the lowest IC50 values in DPPH, ABTS, hydroxyl radical scavenging activity, reducing power, and superoxide dismutase-like activity. Tyrosinase inhibitory activity increased as the concentration increased. All extracts showed higher retardation effects on glucose and bile acid compared to the control; particularly, WSPs showed a similar glucose retardation effect to carboxymethyl cellulose. This study suggests that WSP from C. sinensis can be used as a functional food material.
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KEYWORD
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Cedrela sinensis, polysaccharide, antioxidant, glucose retardation effect, bile acid retardation effect
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